Diurnal changes in the properties of phosphoenolpyruvate carboxylase in Bryophyllum leaves: a possible co valent modification

FEBS Letters

Fewson, C.A.¹  Nimmo, G.A.¹  Wilkins, M.B.¹  Nimmo, H.G.¹ 

[1] Departments of Botany and Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland

关键词: Crassulacean acid metabolism;    Bryophyllum (Kalanchoë) fedtschenkoi;    Diurnal rhythm;    Phosphoenolpyruvate carboxylase;    Covalent modification;    Malate inhibition;    PEPc;    phosphoenolpyruvate carboxylase;   

DOI  :  10.1016/0014-5793(84)80600-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In plants that show Crassulacean acid metabolism, phosphoenolpyruvate carboxylase catalyses the key step of CO₂ fixation at night. We show here that the properties of this enzyme from Bryophyllum fedtschenkoi undergo marked changes between night and day; the night form is much less sensitive to feedback inhibition by malate than is the day form. Incubation of leaves with ³²P_i followed by extraction and immunoprecipitation of phosphoenolpyruvate carboxylase showed that only the night form contained ³²P. This suggests that the activity of the enzyme is controlled by a covalent modification mechanism.

【 授权许可】

Unknown