1H‐NMR of met‐enkephalin in AOT reverse micelles

FEBS Letters

Menegatti, Enea¹  Zetta, Lucia²  Guarneri, Mario¹  De Marco, Antonio² 

[1] Istitulo di Chimica delle Macromolecole del CNR, Via E. Bassini 15/A, 20133 Milano, Italy;Istitutodi Chimica Farmaceutica dell'Università di Ferrara, Via Scandiana 21, 44100 Ferrara, Italy

关键词: Met-enkephalin;    SDS micelle;    AOT reverse micelle;    Shift reagent;    NMR;   

DOI  :  10.1016/0014-5793(84)81235-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹H-NMR spectra of Met-enkephalin dissolved in AOT/isooctane reverse micelles are reported at various temperatures. The NH temperature coefficients are compared with those obtained for the same peptide in normal SDS micelles, in bulk water, and in DMSO. The results show that the opioid molecule undergoes the greatest folding in the reverse micellar system. Shift reagents selectively dissolved in the water pools or in the hydrophobic matrix affect the Phe-4 or the Tyr-1 aromatic resonances, respectively. This suggests that the phenylalanyl sidechain is spatially close to the carboxyl group at the C-terminus, whereas the tyrosyl ring points towards the outer region of the AOT micelles.

【 授权许可】

Unknown