Solubilized monomeric sarcoplasmic reticulum Ca pump protein

FEBS Letters

Martin, Dwight W.¹  Tanford, Charles¹ 

[1] Department of Physiology, Duke University Medical Center, Durham, NC 27710, USA

关键词: Active transport;    Free energy coupling;    Calcium ATPase;    Cu12E8;    dodecyl octaethyleneglycol monoether;    SR;    sarcoplasmic reticulum;   

DOI  :  10.1016/0014-5793(84)81000-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylation (by inorganic phosphate) of sarcoplasmic reticulum Ca pump protein has been studied in a detergent solution in which the protein has been previously shown to exist as a monomer. The course of the reaction is qualitatively similar to that observed for membrane-bound (possibly oligomeric) protein. In particular, the results indicate that alternation between the two principal conformational states of the Ca pump protein persists in the monomeric state, which suggests that the machinery for coupling of ATP hydrolysis to Ca²⁺ transport is intact. There are quantitative differences between monomeric and membrane-bound protein with respect to phosphorylation, but they are not necessarily related to the state of association.

【 授权许可】

Unknown