The effect of phallotoxins on the structure of F‐actin in myosin‐free ghost muscle fibres of rabbit

FEBS Letters

Borovikov, Yu.S.²  Son'kir, B.Ja.¹  Bukatina, A.E.¹ 

[1] Institute of Biophysics, Academy of Sciences of the USSR. Pustchino-na-Okje, Moscow Region, USSR;Institute of Cytology, Academy of Sciences of the USSR, LeningradUSSR

关键词: F-Actin conformational change;    Phallotoxin;    Ghost fiber;    Polarized U V microphotometry;    Ph;    phalloidin;    PhS;    phalloidin-sulfone;    PhSB;    phalloidin-sulfoxide-B;    DTPh;    dithio-phalloidin;    PhSA;    phalloidin-sulfoxide-A;    SPh;    seco-phalloidin;   

DOI  :  10.1016/0014-5793(84)81214-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using polarized UV fluorescent microscopy it has been shown that phallotoxins (phalloidin-sulfone, phalloidin-sulfoxide-B, phalloidin-sulfoxide-A and dithio-phalloidin) cause an increase in tryptophan fluorescence anisotropy of F-actin myofilaments in myosin-free ghost muscle fibres of rabbit. The results obtained are considered to be evidence of conformational changes in F-actin, induced by phallotoxins. These changes are irreversible to a significant extent, which points to a high degree of actin binding to both toxic and nontoxic phallotoxins.

【 授权许可】

Unknown