DNA topoisomerase activities in concanavalin A‐stimulated lymphocytes

FEBS Letters

Lavenot, Catherine²  Vermeersch, Jacqueline²  Mirambeau, Gilles²  der Garabedian, Arsène²  Duguet, Michel²  Taudou, Georges¹ 

[1] Service de Physiopathologie de l'Immunité, Institut Pasteur, 25, rue du Dr. Roux. 75 724 Paris Cedex 15, France;Laboratoired'Enzymologie des Acides Nucleiques, Université Pierre et Marie Curie, 96, Bd. Raspail, 75 006 Paris, France

关键词: Mitogenic agent;    DNA replication;    Lymphocyte;    Topoisomerase;    Con A;    concanavalin A;    EGF;    epidermal growth factor;   

DOI  :  10.1016/0014-5793(84)81212-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Topoisomerase activities have been measured in nuclear extracts of concanavalin A-stimulated lymphocytes. In parallel with the wave of DNA synthesis, type II topoisomerase activity was considerably increased. After 72 h treatment, this activity was stimulated approx. 20-fold over the activity in untreated cells. In contrast, type I topoisomerase was poorly stimulated after 24 h treatment, and 4-5-fold after 72 h. These findings, together with our previous results on regenerating rat liver, suggest a major role of topoisomerase II in DNA replication.

【 授权许可】

Unknown