FEBS Letters | |
Bending of smooth muscle myosin rod | |
Cross, R.A.1  | |
[1] Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020 Salzburg, Austria | |
关键词: Smooth muscle myosin; Myosin rod; Myosin hinge; LMM; light meromyosin; SF 2; heavy meromyosin subfragment 2; PAGE; polyacrylamide gel electrophoresis; | |
DOI : 10.1016/0014-5793(84)80940-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Electron microscopy of mammalian smooth muscle myosin rods showed them to be 153 ± 7nm (SD) long, and to bend sharply (> 90°) but infrequently, and pH independently (range 6.5–9.5), at a single site 45 ± 4 nm from one end of the molecule. Light meromyosin (LMM) preparations were 99 ± 10 nm long, and showed no bends. Intrinsic viscosity vs temperature plots for rods and LMM indicated that neither fragment changed in flexibility in the range 4–40° C. Peptide mapping in the presence and absence of SDS established that the proteolytic susceptibility of the hinge at the N terminus of LMM reflects the presence of locally different structure, and not simply a clustering of susceptible residues. The isolated smooth muscle myosin rod thus contains only a single hinge, having significant stiffness, and lacks the second bend seen under certain conditions in the intact molecule.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020285968ZK.pdf | 1823KB | download |