Antibodies to guanosine

FEBS Letters

Jacob, T.M.¹  Iswari, S.¹ 

[1] Department of Biochemistry, and ICMR Centre for Cell Biology and Genetics, Indian Institute of Science, Bangalore-560012, India

关键词: Guanosine;    Antibody binding;    G;    guanosine;    I;    inosine;    A;    adenosine;    C;    cytosine;    U;    uracil;    pG;    guanosine 5'-phosphate;    Qox-red;    guanoing oxidized with NaIO4 and reduced with NaBH4;    similarly Aox-red;    Uox-red;    Cox-red;    Iox-red;    ssDNA;    single-stranded DNA;    dsDNA;    double-stranded DNA;   

DOI  :  10.1016/0014-5793(84)80908-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine serum albumin conjugates of guanosine prepared by the periodate method was used as immunogen to elicit guanosine antibodies in rabbits. The specificities of the antibodies were studied by the inhibition of their binding to [³H]G^ox-red, [³²P]DNA and [³H]RNA by related non-radioactive compounds. A population of antibodies is specific to G^ox-red with an average association constant of around 10⁷M⁻¹ at 4°C. There are a population of antibodies which bind to [³²P]ssDNA and [³H]RNA specifically at guanosine residues. RNA binding antibodies were separated into two populations by affinity chromatography.

【 授权许可】

Unknown