Inactivation and reactivation of light‐triggered ATP hydrolysis on the chloroplast coupling factor

FEBS Letters

Komatsu-Takaki, Mizuho¹ 

[1] Department of Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, 199-01, Japan

关键词: ATP hydrolysis;    Activation;    Energized state;    P1 effect;    NH4Cl effect;    Chloroplast;   

DOI  :  10.1016/0014-5793(84)80783-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Decay of light-triggered ATP hydrolysis in the dark was diminished with a decrease in chloroplast concentration. The enhancing effect of NH₄Cl on ATP hydrolysis decreased with dark time. The decrease was much faster than that in ATP hydrolysis activity. The NH₄Cl effect increased with ATP preincubation time. Reactivation of ATP hydrolysis occurred with the progress of ATP hydrolysis. P_i enhanced the activation remarkably. These results suggest that ATP hydrolysis produces some energized state, which stimulates NH₄C1 effect and makes coupling factor active in the presence of P_i and that to keep coupling factor active, energy is not necessarily needed.

【 授权许可】

Unknown