H1 histone, polylysine and spermine facilitate nucleosome assembly in vitro

FEBS Letters

Bogdanova, E.S.¹ 

[1] Institute of Molecular Genetics, USSR Academy of Sciences, Kurchatov Sq., Moscow 123182, USSR

关键词: Nucleosome assembly;    Assembly factor;    H1 histone;    Polylysine;    Spermine;   

DOI  :  10.1016/0014-5793(84)80760-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nucleosome formation has been studied in a system containing relaxed Col El DNA, core histones and an extract of Drosophila embryos. The formation of nucleosomes was established by the introduction of supercoils into DNA. The degree of DNA supercoiling was shown to be higher if nucleosomes were assem-bled in the presence of the H1 histone, polylysine (M _r 20000) or spermine. These agents do not stimulate relaxation and are the more effective the earlier they are added to the reaction. Thus, the H1 histone, polylysine and spermine facilitate nucleosome assembly in vitro.

【 授权许可】

Unknown