Cytochrome b reduction by hexaammineruthenium in mitochondria and submitochondrial particles

FEBS Letters

Kunz, Wolfram S.¹  Konstantinov, Alexander² 

[1] Institut für Physiologische Chemie, Medizinische Akademie Magdeburg, Leipziger Str. 44, Magdeburg, DDR;A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR

关键词: Respiratory chain;    Redox center topography;    Cytochrome;    b;    Hexaamineruthenium;    Q-cycle;    SMP;    submitochondrial particles;    RuAm;    hexaammineruthenium;   

DOI  :  10.1016/0014-5793(84)80578-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the presence of ascorbate, hexaamineruthenium mediates rapid reduction of cytochrome b-562 in submitochondrial particles but not in mitochondria. The reaction is obsreved in the combined presence of antimycin (or funiculosin) and myxothiazol, which implies direct interaction of Ru(NH₃)²⁺ ₆ with b cytochrome(s). We assume that contrary to previous conclusions (Case and Leigh (1976) Biochem. J., 160, 769-783) redox centre of at least one of the oxidized cytochromes b, most probably of b-562, is exposed to the M-aqueous phase.

【 授权许可】

Unknown