The complete amino‐acid sequence of anglerfish somatostatin‐28 II

FEBS Letters

Chang, Jui-Yoa²  Cohen, Paul¹  Morel, Alain¹ 

[1] Groupe de Neurobiochimie Cellulaire et Moléculaire, Unité Associée au CNRS No. 554, Université Pierre et Marie Curie, 96 boulevard Raspail. 75006 Paris, France;Ciba-Geigy Ltd., CH-4002 Basel, Switzerland

关键词: Amino-acid sequencing;    Anglerfish;    Somatostatin-28 II;    Somatostatin-14 I;    Pancreatic islet;    DABSCI;    dimethylaminoazobenzene sulphonyl chloride;    DABITC;    4-N;    N-dimethylamino-benzene 4'isothiocyanate;    PITC;    phenylisothiocyanate;    HPLC;    High Performance Liquid Chromatography;    PTH;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(84)80561-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new somatostatin-28 has been isolated from the Teleostean fish (Lophius piscatorius) Brockmann organs. Determination of its aminoacid sequence indicates that it corresponds to an octacosapeptide containing in its C-terminal end the Tyr-7 Gly-10 derivative of somatostatin-14 I. This structure is in agreement with the one predicted by Hobart et al. (Nature (1980) 288, 137-141) from a cDNA nucleotide sequence. It demonstrates that, since the corresponding somatostatin-14 II cannot be detected in this organ, S-28 II is a terminal product of prosomatostatin II processing in anglerfish pancreatic islets.

【 授权许可】

Unknown