Calcitonin mRNA activity in young obese (fa/fa) Zucker rats

FEBS Letters

Garel, J.-M.¹  Segond, N.²  Jullienne, A.²  Tahri, E.H.¹ 

[1] GroupeHormones du Calcium et Développement, Université Pierre et Marie Curie, 4 Place Jussieu, 75230 Parix Cédex 05, France;Unité 113 de L'INSERM, CHU Saint-Antoine, 27 Rue Chaligny, 75571 Paris Cédex 12 France

关键词: mRNA activity;    Calcitonin;    Young obese rat;    Zucker rat;   

DOI  :  10.1016/0014-5793(84)81083-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6) in obese animals, but plasma phosphate was unchanged. Plasma CT levels measured by radioimmunoassay (RIA) were significantly decreased in fatty (0.50 ± 0.03 vs 0.68 ± 0.03 in Leans; P < 0.001), but thyroidal hormone content was not different between Lean and fatty rats (68.7 ± 5.1 in Leans vs 60.5 ± 3.6 in fatty rats). mRNA was extracted from 10 thyroids, and translated in a rabbit reticulocyte lysate (NEN) in the presence of [³⁵S]methionine. After polyacrylamide gel electrophoresis, specific immunoprecipitates were autoradiographied and quantified by integration. A 50% decrease in translatable CT mRNA was observed in fatty rats. In basal conditions, the biosynthetic activity of C cells in obese rats correlates with the secretion rate of the hormone in the face of unchanged thyroidal CT contents.

【 授权许可】

Unknown