Calmodulin activates adenylate cyclase from rabbit heart plasma membranes

FEBS Letters

Tkachuk, V.A.¹  Panchenko, M.P.¹ 

[1]Institute of Experimental Cardiology, Cardiology Research Center of the USSR, Academy of Medical Sciences, 3rd Cherepkovskaya street 15A, Moscow 121552, USSR

关键词: Calmodulin;    Adenylate cyclase;    Cardiac light sarcolemma;    Guanine nucleotide;    Ca2+;    Gpp(NH)p;    guanosine 5'-(β;    γ-imido)triphosphate;   

DOI  :  10.1016/0014-5793(84)81076-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It was shown that calmodulin (CM) activates the adenylate cyclase (AC) of rabbit heart light sarcolemma in the presence of micromolar free Ca²⁺ concentrations and this effect is blocked by trifluoroperazine and troponin I. GTP (in the presence of isoproterenol) and Gpp(NH)p are able to increase the CM-dependent activity of enzyme. It was concluded that there is no special CM-dependent ‘form’ of AC in the heart and the common catalytic component of AC can be regulated both by CM and guanine nucleotide-binding regulatory component (N-protein). In the presence of Ca²⁺ and guanine nucleotide heart AC exists as a complex: CM-catalytic component-N-protein.

【 授权许可】

Unknown