Lipoxygenase‐mediated production of superoxide anion in senescing plant tissue

FEBS Letters

Lynch, Daniel V.¹  Thompson, John E.¹ 

[1] Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada

关键词: Senescence;    Lipoxygenase;    Superoxide anion;    Tiron;    U28938;    4-(1-methylethyl)benzynecarbohydrazonoyl chloride N-phenyl;    CHES;    2-[N-cyclohexylamino]ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(84)81057-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lipoxygenase activity and superoxide (O^.− ₂) production by microsomal membranes and cytosol from bean cotyledons increased in parallel as senescence progressed. Superoxide production was heat denaturable and dependent on the availability of linoleate, the substrate for lipoxygenase. The specific inhibitor of lipoxygenase, U28938, caused a parallel reduction in enzyme activity and the formation of O⁻ ₂. These observations demonstrate that lipoxygenase activity mediates the formation of superoxide anion, and support the contention that membrane senescence is attributable to a sequence of reactions in which lipasederived fatty acids are utilized by lipoxygenase to generate O⁻ ₂ and hydroperoxides.

【 授权许可】

Unknown