Thymidylate synthetase‐positive and ‐negative murine mammary FM3A carcinoma cells as a useful system for detecting thymidylate synthetase inhibitors

FEBS Letters

Balzarini, J.²  de Clercq, E.²  Ayusawa, D.¹  Seno, T.¹ 

[1] Department of Immunology and Virology, Saitama Cancer Center Research Institute, Ina-machi, Saitama-ken 367, Japan;Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium

关键词: Murine mammary;    FM3A carcinoma;    Thymidylate synthetase;    5-Substituted;    2'-deoxyuridine analog;   

DOI  :  10.1016/0014-5793(84)81052-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS⁻ carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/TS⁻ cells, and their inhibitory effects on FM3A/O cell growth should be readily reversed by exogenous dThd within the concentration range of 5–20 μM.

【 授权许可】

Unknown