Echinocandin inhibition of 1,3‐β‐D‐glucan synthase from Candida albicans

FEBS Letters

Sawistowska-Schröder, E.T.¹  Kerridge, D.¹  Perry, H.¹ 

[1] Sub-Department of Chemical Microbiology, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: Candida albicans;    Antibiotic;    Echinocandin;    1;    3-β-D-Glucan synthase;    1;    3-β-D-Glucanase GTP;   

DOI  :  10.1016/0014-5793(84)81032-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cyclic peptide antibiotic echinocandin was found to inhibit 1,3-β-D-glucan synthase activity present in a mixed membrane fraction from Candida albicans. Addition of antibiotic reduced the V _max of the enzyme, but the K _m was unaffected. GTP stimulated enzyme activity ~ 4-fold, but did not affect the percentage inhibition of the enzyme by echinocandin. Treatment of the reaction products with α-amylase and β-glucanase confirmed that the polymer synthesised was 1,3-β-D-glucan, not glycogen.

【 授权许可】

Unknown