期刊论文详细信息
FEBS Letters | |
Echinocandin inhibition of 1,3‐β‐D‐glucan synthase from Candida albicans | |
Sawistowska-Schröder, E.T.1  Kerridge, D.1  Perry, H.1  | |
[1] Sub-Department of Chemical Microbiology, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England | |
关键词: Candida albicans; Antibiotic; Echinocandin; 1; 3-β-D-Glucan synthase; 1; 3-β-D-Glucanase GTP; | |
DOI : 10.1016/0014-5793(84)81032-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The cyclic peptide antibiotic echinocandin was found to inhibit 1,3-β-D-glucan synthase activity present in a mixed membrane fraction from Candida albicans. Addition of antibiotic reduced the V max of the enzyme, but the K m was unaffected. GTP stimulated enzyme activity ~ 4-fold, but did not affect the percentage inhibition of the enzyme by echinocandin. Treatment of the reaction products with α-amylase and β-glucanase confirmed that the polymer synthesised was 1,3-β-D-glucan, not glycogen.
【 授权许可】
Unknown
【 预 览 】
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