Isolation of the gene encoding pilin of Bacteroides nodosus (strain 198), the causal organism of ovine footrot

FEBS Letters

Stewart, D.J.¹  Elleman, T.C.²  Emery, D.L.¹  Hoyne, P.A.²  Clark, B.L.¹ 

[1] CSIRO Division of Animal Health, Park Drive, Parkville, Victoria 3052, Australia;CSIRO Division of Protein Chemistry, 343 Royal Parade, Parkville, Victoria 3052 Australia

关键词: Pilin;    Bacteroides nodosus;    Footrot;    Cloning;    PAGE;    polyacrylamide gel electrophoresis;    kbp;    kilobase pair;   

DOI  :  10.1016/0014-5793(84)81026-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene for pilin, the monomeric protein subunit from which the pilus of Bacteroides nodosus is constructed, has been isolated. Isolation was achieved by cloning the fragmented genome of B. nodosus in Escherichia coli RR1 using the plasmid vector pBR322. Pilin-producing colonies were identified by screening with a colony immunoassay using antiserum from a sheep immunized against purified pili from B. nodosus strain 198, and were further characterized by immunoblot analysis. Final confirmation of the presence of the pilin gene was by nucleotide sequence data which translated to the known pilin amino acid sequence.

【 授权许可】

Unknown