Biosynthesis of intestinal microvillar proteins

FEBS Letters

Cowell, Gillian M.¹  Danielsen, E.Michael¹ 

[1] Department of Biochemistry C, The Panum Institute, University of Copenhagen, 3 Blegdamsvej, DK-2200 Copenhagen N, Denmark

关键词: Microvillus;    Biosynthesis;    Cytoskeleton;    Membrane protein;    Small intestine;   

DOI  :  10.1016/0014-5793(84)81147-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using alkaline extraction to separate cytoskeletal and membrane proteins of intestinal microvilli, the kinetics of assembly of these two microvillar protein compartments was studied by pulse-chase labelling of pig small intestinal mucosal explants, kept in organ culture. Following a 10 min pulse of [³⁵S]methionine, the membrane proteins did not appear in the microvillar fraction until after 40–60 min of chase. In contrast, the cytoskeletal components, of which the 110-kDa protein and villin were immunologically identified, were expressed in the microvillar fraction immediately after the 10 min pulse. These different kinetics of appearance indicate that the two microvillar protein compartments have separate mechanisms of biosynthesis and microvillar expression.

【 授权许可】

Unknown