Evidence for cytochrome bc 1 complex involvement in nitrite reduction in a photodenitrifier, Rhodopseudomonas sphaeroides forma sp. denitrificans

FEBS Letters

Urata, Katsuro¹  Satoh, Toshio¹ 

[1] Department of Biology, Faculty of Science, Tokyo Metropolitan University, Fukazawa 2-1-1, Setagaya-ku, Tokyo 158, Japan

关键词: Cytochrome;    bc1;    complex;    Dissimilatory nitrite reduction;    Denitrification;    Rhodopseudomonas sphaeroides;   

DOI  :  10.1016/0014-5793(84)81126-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytochrome bc ₁ complex which has a quinol : cytochrome c ₂ oxidoreductase activity was isolated from a photodenitrifier, Rhodopseudomonas sphaeroides forma sp. denitrificans. When duroquinol (tetramethyl-p-benzoquinol) was used as the electron donor, maximum nitrite reduction activity was obtained in an anaerobic mixture containing the isolated cytochrome bc ₁ complex, nitrite reductase and cytochrome c ₂ Nitrite reduction in chromatophore membranes, as well as in the reconstituted system, was inhibited in the presence of antimycin A. No nitrite reduction occurred in the absence of cytochrome c ₂. These results indicated that the cytochrome bc ₁ complex involved in photosynthetic electron transfer also donates reducing equivalents to the dissimilatory nitrite reductase via cytochrome c ₂ in this photodenitrifier.

【 授权许可】

Unknown