Immunological identification of four different polypeptides in ‘subunit VII’ of mammalian cytochrome c oxidase

FEBS Letters

Kuhn-Nentwig, Lucia¹  Kadenbach, B.¹ 

[1]Biochemie, Fb Chemie der Philipps-Universität, Hans-Meerwein Straße, D-3550 Marburg, FRG

关键词: Cytochrome c oxidase;    Immunoblotting;    Subunit composition;    Monospecific antiserum;    SDS-gel electrophoresis;   

DOI  :  10.1016/0014-5793(84)81123-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat liver cytochrome c oxidase was separated by SDS-gel electrophoresis into 13 polypeptide bands. Monospecific antisera against the isolated polypeptides VIIa, VIIb and VIIc were raised in rabbits. Cytochrome c oxidase was blotted on nitrocellulose and incubated with the antisera. The antisera reacted only with their corresponding polypeptides, indicating no immunological relationship between polypeptides VIla, VIIb and VIIc. The data also exclude that these polypeptides are proteolytic breakdown products of larger subunits.

【 授权许可】

Unknown