Limulus ventral photoreceptors contain a homologue of the α‐subunit of mammalian Ns

FEBS Letters

Kaupp, U.B.¹  Brown, J.E.¹  Malbon, Craig C.² 

[1] Department of Pharmacological Sciences and Department of Physiology and Biophysics, The State University of New York, Stony Brook, NY 11794-8651, USA, Schwerpunkt Biophysik, Universität Osnabrück, D-4500 Osnabrück, FRG and The Marine Biological Laboratory, Woods Hole, MA 02543, USA

关键词: Limulus Photoreceptor Cholera toxin ADP-ribosylation Stimulatory guanine nucleotide-binding regulatory component;   

DOI  :  10.1016/0014-5793(84)80880-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Membranes from ventral photoreceptors of Limuluswere incubated with cholera toxin and [³²P]NAD⁺. Cholera toxin catalyzes a specific ADP-ribosylation of a 43-kDa peptide from Limulus ventral photoreceptors. Possible homologies between the 43-kDa peptide of Limulus and the α-subunits of mammalian stimulatory, guanine nucleotide-binding regulatory component of adenylate cyclase (N_s) were investigated by comparing the electrophoretic patterns of proteolytic fragments derived from each of these peptides that are radiolabeled by [³²P]NAD⁺ and cholera toxin. Evidence is provided for structural homology between this invertebrate peptide and mammalian N_s.

【 授权许可】

Unknown