DNA rearrangements generating artificial promoters

FEBS Letters

Gragerov, A.I.²  Korobko, V.G.¹  Smirnov, O.Yu.²  Mekhedov, S.L.²  Chuvpilo, S.A.¹  Nikiforov, V.G.² 

[1] Shemyakm Institute of Bioorganic Chemistry, USSR Academy of Sciences, Vavilov street 32, 117988 Moscow, USSR;Institute of Molecular Genetics, USSR Academy of Sciences, Kurchatov sq.46, 123182 MoscowUSSR

关键词: Pribnow box ‘−35’ region S1-mapping;   

DOI  :  10.1016/0014-5793(84)80874-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The promoter-cloning plasmid pBRH4 (a derivative of pBR322 with a partially deleted promoter of the tet gene) is shown to contain a sequence which is located near the EcoRI site and can operate as an effective Pribnow box, but is not the remainder of the deletion-inactivated tet promoter of pBR322. If there is a sequence homologous to the ‘−35’ promoter region at the border of the DNA fragment inserted at the EcoRI site, then a compound promoter arises and activates the tet gene. Point mutations in the nonfunctional −35 region of pBRH4 also activate the cryptic Pribnow box. Several compound promoters were obtained through deleting small portions of DNA around the HindIII site of pBR322; the deletions moved various sequences that could operate as Pribnow boxes towards the −35 region of the tet promoter.

【 授权许可】

Unknown