FEBS Letters | |
DNA rearrangements generating artificial promoters | |
Gragerov, A.I.2  Korobko, V.G.1  Smirnov, O.Yu.2  Mekhedov, S.L.2  Chuvpilo, S.A.1  Nikiforov, V.G.2  | |
[1] Shemyakm Institute of Bioorganic Chemistry, USSR Academy of Sciences, Vavilov street 32, 117988 Moscow, USSR;Institute of Molecular Genetics, USSR Academy of Sciences, Kurchatov sq.46, 123182 MoscowUSSR | |
关键词: Pribnow box ‘−35’ region S1-mapping; | |
DOI : 10.1016/0014-5793(84)80874-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The promoter-cloning plasmid pBRH4 (a derivative of pBR322 with a partially deleted promoter of the tet gene) is shown to contain a sequence which is located near the EcoRI site and can operate as an effective Pribnow box, but is not the remainder of the deletion-inactivated tet promoter of pBR322. If there is a sequence homologous to the ‘−35’ promoter region at the border of the DNA fragment inserted at the EcoRI site, then a compound promoter arises and activates the tet gene. Point mutations in the nonfunctional −35 region of pBRH4 also activate the cryptic Pribnow box. Several compound promoters were obtained through deleting small portions of DNA around the HindIII site of pBR322; the deletions moved various sequences that could operate as Pribnow boxes towards the −35 region of the tet promoter.
【 授权许可】
Unknown
【 预 览 】
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