Solubilization and partial purification of an enzyme converting 1‐aminocyclopropane‐l‐carboxylic acid to ethylene in plants

FEBS Letters

Adam, Zach¹  Mayak, Shimon¹ 

[1] Department of Ornamental Horticulture, The Hebrew University of Jerusalem, Rehovot 76100, Israel

关键词: Ethylene 1-Aminocyclopropane-1-carboxylic acid Solubilization;    ACC;    1-aminocyclopropane-1-carboxylic acid;    Ches;    N-cyclohexylaminoethanesulfonic acid;    Epps;    N-2-hydroxyethylpiperazinepropanesulfonic acid;    Mes;    N-morpholinoethanesulfonic acid;    Tes;    N-trishydroxymethylmethyl-2-aminoethanesulfonic acid;   

DOI  :  10.1016/0014-5793(84)80870-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The membrane-bound enzyme capable of catalyzing the conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene can be solubilized by treatment with detergent. The non-ionic detergent Nonidet P-40, at an optimum concentration of 0.2% and a protein : detergent ratio of 1:2, was found to be an effective solubilizer of the conversion activity. The solubilized enzyme resembles the membrane-bound enzyme with respect to heat sensitivity, pH dependence, Mn^{2 +} stimulation and Co^{2 +} inhibition. The enzyme can be further purified by means of ion-exchange chromatography with 12-fold increase in specific activity.

【 授权许可】

Unknown