Monoclonal antibodies which prevent experimental lung metastases

FEBS Letters

Schirrmacher, V.²  Birchmeier, W.¹  Vollmers, H.P.¹  Imhof, B.A.¹  Waller, C.A.²  Braun, S.¹ 

[1] Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, D-7400 TübingenF.R.G.;Institut für Immunologie und Genetik, Deutsches Krebsforschungszentrum, D-6900 Heidelberg, FRG

关键词: Monoclonal antibody Metastasis Cell adhesion Laminin Cancer therapy Extracellular matrix;   

DOI  :  10.1016/0014-5793(84)80863-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cellular adhesion is important during metastasis, as metastatic cells must escape from the primary site into lymph and blood systems, there to adhere specifically to sites in distant organs. We have recently selected monoclonal antibodies which prevent adherence of B 16 mouse melanoma cells to tissue culture dishes, and also markedly reduce experimental lung metastasis in mice when injected before or with the tumour cells. Here, we investigated which step in the metastatic process may be affected by the antibodies. The possible inhibitory effect of antibody on tumour cell adherence to vascular endothelial monolayers and to purified components of the underlying extracellular matrix-fibronectin, laminin and collagen type IV - was studied using in vitro assays. We found that the antibodies significantly blocked attachment to laminin, suggesting that specific basement membrane components play an important role in attracting or otherwise modifying the behaviour of metastatic tumour cells.

【 授权许可】

Unknown