Sidedness of plant plasma membrane vesicles purified by partitioning in aqueous two‐phase systems

FEBS Letters

Widell, S.¹  Lundborg, T.²  Kjellbom, P.³  Larsson, C.³ 

[1] Department of Plant Physiology, University of Lund, PO Box 7007, S-220 07 Lund, Sweden;Department of Crop Genetics and Breeding, The Swedish University of Agricultural Sciences, Herman Ehles väg 2, S-268 00 Svalöv, Sweden;Department of Biochemistry, University of Lund, PO Box 740, PO Box 740, S-220 07 Lund, Sweden

关键词: Plant plasma membrane vesicle;    Sidedness;    Enzyme latency;    K+-stimulated Mg2+-dependent ATPase;    Glucan synthetase II;    Two-phase system;   

DOI  :  10.1016/0014-5793(84)80502-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Preparations of plant plasma membrane vesicles were obtained by partition in dextran-polyethylene glycol two-phase systems. By this procedure particles are separated according to their surface properties, and an iso-osmotic environment is maintained throughout. The vesicles thus produced are right-side-out and sealed, as measured by enzyme [(K₊ + Mg²⁺)-ATPase and glucan synthetase II] latency on addition of Triton X-100.

【 授权许可】

Unknown