Allosteric regulation of NAD(NADP)‐dependent glyceraldehyde‐3‐phosphate dehydrogenase from Chlorella by α‐amino acids, dithiothreitol and ATP

FEBS Letters

Tomova, Nicolina G.¹  Georgieva, Mimi A.¹  Krusteva, Nicolina G.¹ 

[1] Institute of Organic Chemistry and Centre of Phytochemistry, Bulgarian Academy of Sciences, ul. Akad. G. Bonchev block 11, Sofia 1113, Bulgaria

关键词: Allosteric regulation;    Glyceraldehyde-3-phosphate dehydrogenase;    Chlorella;    α-Amino acid;    Dithiothreitol;    ATP;    GAPD;    D-glyceraldehyde-3-phosphate dehydrogenase;    Hepes;    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid;    DTT;    dithiothreitol;   

DOI  :  10.1016/0014-5793(84)80474-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Amino acids (glycine, serine, histidine, aspartic acid and cysteine) and dithiothreitol (DTT) have been shown to activate both activities of the NAD(NADP)-dependent glyceraldehyde-3-phosphate dehydrogenase from Chlorella. The activation is allosteric and reaches 200–700%. The Hill coefficient values are close to 2 with all activators. ATP activates NADP-dependent but inhibits NAD-dependent activity, n _app and K values being the same for both enzyme activities. In this case positive cooperativity is also observed (n _app = 2.2). The present findings reveal the possible regulation of GAPD function in Chlorella with each of the coenzymes.

【 授权许可】

Unknown