Solubilization in high yield of opioid receptors retaining high‐affinity delta, mu and kappa binding sites

FEBS Letters

Barnard, Eric A.¹  Demoliou-Mason, Catherine D.¹ 

[1] Department of Biochemistry, Imperial College of Science and Technology, London SW7 2AZ, England

关键词: Opioid receptor;    Receptor solubilization;    Subtype;   

DOI  :  10.1016/0014-5793(84)81348-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding sites for opiates (agonist and antagonist) and opioid peptides can be solubilized from rat brain membranes with digitonin in the presence of Mg²⁺ (10 mM). High affinity and high capacity binding to the soluble δ, μ, and κ receptors is obtainable when the membranes are treated in Mg²⁺ (30°C, 60 min) prior to solubilization. The yields of solubilized binding sites extracted with digitonin, 40–90%, are higher than those obtained from Mg²⁺-pretreated membranes with other detergents commonly used for receptor solubilization. The stability of the digitonin-soluble opioid receptor at room temperature makes it useful for purification and characterization.

【 授权许可】

Unknown