In vitro studies on origin and site of action of enzyme activity responsible for conversion of human proapoprotein A‐I into apoprotein A‐I

FEBS Letters

Louis, Havekes¹  Kooistra, Teake¹  Victor, van Hinsbergh¹  Herman Jan, Kempen¹ 

[1] Gaubius Institute, Health Research Division TNO, Herenstraat 5d, 2313 AD Leiden, The Netherlands

关键词: Human proapolipoprotein A-I;    Hep G2 cell;    Endothelial cell;    HDL metabolism;    apo;    apolipoprotein;    HDL;    LDL and VLDL;    high density;    low density and very low density lipoprotein;    respectively;    LPDS;    lipoprotein-depleted serum;    ECGS;    endothelial cell growth supplement;    EGF;    epidermal growth factor;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(84)81379-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein. No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca²⁺/Mg²⁺-dependent enzyme which is present on the surface of plasma lipoprotein particles, endothelial cells and Hep G2 cells, and is probably synthesized by Hep G2 cells.

【 授权许可】

Unknown