Distribution of non‐enzymatically bound glucose in in vivo and in vitro glycosylated type I collagen molecules

FEBS Letters

Muh, Jean-Pierre¹  Guitton, Jean-Dominique¹  Le Pape, Alain¹ 

[1] ERA CNRS 697, Faculté de Médecine, 37032 Tours Cédex, France

关键词: Collagen;    Nonenzymatic glycosylation;    Diabetes;    Glucose localization;    CNBr cleavage;    Fluorography;   

DOI  :  10.1016/0014-5793(84)81361-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Non-enzymatic glycosylation of collagen occurs both in vivo during diabetes and in vitro after incubation with glucose. Glycosylated collagen exhibits altered physicochemical and biological properties which could explain some of the complications of diabetes. To provide a mechanistic explanation of this modification the localization of bound glucose was investigated using NaB[³H]H₄ reduction and CNBr cleavage. Glucose fixation is distributed mainly on the α₁CB6 peptide after in vitro glycosylation whereas this distribution occurs less specifically during diabetes. It is concluded that fibrillogenesis alteration of in vitro glycosylated collagen is related to glucose fixation on free ϵNH₂ sites normally implied in intermolecular interactions.

【 授权许可】

Unknown