Two protons are pumped from the mitochondrial matrix per electron transferred between NADH and ubiquinone

FEBS Letters

Wikström, Mårten¹ 

[1] Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10A, SF-00170 Helsinki 17, Finland

关键词: H+ translocation;    H+/e− stoichiometry;    Mitochondria;    Energy conservation;    NEM;    N-ethylmaleimide;    nig;    nigericin;    NR;    neutral red;    Pi;    inoganic phosphate;    Q;    ubiquinone;    QH2;    ubiquinol;    q+/e−;    ratio of translocation of electrical charge equivalents per transferred electron;    val;    valinomycin;   

DOI  :  10.1016/0014-5793(84)80338-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The spectrophotometric indicators neutral red and safranine were used to determine the relative H⁺/e⁻ ratios of proton uptake from the mitochondrial matrix, and the q⁺/e⁻ ratios of electrical charge translocation, during oxidation of β-OH-butyrate and succinate by ferricyanide in rat liver mitochondria. With β-OH-butyrate both ratios were higher than with succinate by a factor close to 3.0. Since there is full agreement that H⁺/e⁻ of proton uptake and q⁺/e⁻ of charge translocation are both equal to unity for oxidation of succinate (or ubiquinol) by ferricytochrome c, the corresponding ratios for oxidation of NADH by ubiquinone and cytochrome c are near 2.0 and 3.0, respectively.

【 授权许可】

Unknown