Detection of two DCCD‐binding components in the envelope membrane of H. halobium

FEBS Letters

Konishi, Tetsuya¹  Murakami, Naoyuki¹ 

[1] Department of Radiochemistry-Biology, Niigata College of Pharmacy, 5829 Kamishin'ei, Niigata 950-21, Japan

关键词: DCCD-binding protein;    H. halobium envelope membrane;    H+ transport;    [14C]DCCD;    DCCD;    N;    N-dicyclohexylcarbodiimide;    HPLC;    high-performance liquid chromatography;    SDS—PAGE;    SDS-polyacrylamide gel electrophoresis;    Δψ;    membrane electrical potential;    Pipes;    piperazine-N;    N-bis(2-ethanesulfonic acid);    V t;    bed volume;   

DOI  :  10.1016/0014-5793(84)80334-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

From labeling studies using [¹⁴C]DCCD, the presence of two distinct DCCD-binding sites was proved in halobacterial membrane, whose binding constants are 0.03 and 0.08 nmol DCCD/mg envelope protein, respectively. HPLC and SDS—PAGE analysis revealed that the radioactivity bound to these sites migrated with apparent molecular masses of 45 and 10.2 kDa protein components, respectively.

【 授权许可】

Unknown