期刊论文详细信息
FEBS Letters | |
Purification and characterization of ubiquitin from mammalian testis | |
Menezo, Y.1  Loir, M.2  Muh, J.P.3  Caraty, A.2  Lanneau, M.2  Sautiere, P.4  | |
[1] INSA, Lab. Biologie 406, 69621 Villeurbanne, France;INRA Physiologie de la Reproduction, 37380 Nouzilly, France;ERA 697, CNRS, Faculté Médecine, 37032 Tours, France;U 124 INSERM, Place de Verdun, 59000 Lille, France | |
关键词: Ubiquitin; Radioimmunoassay; Amino acid analysis; Gel filtration chromatography; Mammalian testis; | |
DOI : 10.1016/0014-5793(84)80318-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH2-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved methods for radioimmunoassay of ubiquitin in tissue extracts are also discussed.
【 授权许可】
Unknown
【 预 览 】
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