Purification and characterization of ubiquitin from mammalian testis

FEBS Letters

Menezo, Y.¹  Loir, M.²  Muh, J.P.³  Caraty, A.²  Lanneau, M.²  Sautiere, P.⁴ 

[1] INSA, Lab. Biologie 406, 69621 Villeurbanne, France;INRA Physiologie de la Reproduction, 37380 Nouzilly, France;ERA 697, CNRS, Faculté Médecine, 37032 Tours, France;U 124 INSERM, Place de Verdun, 59000 Lille, France

关键词: Ubiquitin;    Radioimmunoassay;    Amino acid analysis;    Gel filtration chromatography;    Mammalian testis;   

DOI  :  10.1016/0014-5793(84)80318-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ubiquitin was extracted from testis of 4 mammals and purified to homogeneity by gel filtration chromatography. Amino acid compositions and NH₂-terminal sequences were found to be identical in the 4 species and with calf thymus ubiquitin. Ubiquitin conformation was shown to be very sensitive to oxidation. Improved methods for radioimmunoassay of ubiquitin in tissue extracts are also discussed.

【 授权许可】

Unknown