ATP production coupled to the denitrification of nitrate in Rhizobium japonicum, grown in cultures and in bacteroids from Glycine max

FEBS Letters

Bhandari, Basant¹  Naik, M.S.¹  Nicholas, D.J.D.¹ 

[1] Department of Agricultural Biochemistry, Waite Agricultural Research Institute, The University of Adelaide, Glen Osmond, SA 5064, Australia

关键词: Rhizobium japonicum;    Bacteroid;    Denitrification;    ATP production;    BV;    benzyl viologen;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DCPIP;    2;    6-dichlorophenolindophenol;    DNP;    2;    4-dinitrophenol;    HOQNO;    2-heptyl-4-hydroxyquinoline N-oxide;    MV;    methyl viologen;    PMS;    phenazine methosulfate;   

DOI  :  10.1016/0014-5793(84)80271-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The denitrification of nitrate in membrane fractions of Rhizobium japonicum CC705 grown in culture solutions with nitrate and those from bacteroids of Glycine max was coupled to ATP production. The ATP/2e⁻ ratios for electron transfer from succinate and NADH to NO⁻ ₃ and O₂ in membrane fractions of R. japonicum grown with nitrate were: succinate to O₂, 2.0; succinate to nitrate, 1; NADH to O₂, 3; NADH to nitrate, 2. In membrane fractions of bacteroids the value for succinate to nitrate was also 1.

【 授权许可】

Unknown