Length of myosin rod and its proteolytic fragments determined by electron microscopy

FEBS Letters

Edwards, Pat¹  Stewart, Murray¹ 

[1] MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB22QH, England

关键词: Myosin;    Myosin rod;    Light meromyosin;    Myosin subfragment-2;    Molecular length;   

DOI  :  10.1016/0014-5793(84)80209-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mean length of rabbit myosin chymotryptic rod in electron micrographs of unidirectionally shadowed preparations was 154 nm. Tryptic light meromyosin prepared from this rod had a mean length of 86 nm whereas long and short S2 measured 62 and 43 nm, respectively. The combined length of light meromyosin and long S2 appeared to be less than that of rod, which indicated that possibly some material was lost on digestion. Molecular lengths agreed with sedimentation equilibrium M _r values but were lower than those indicated by gel electrophoresis.

【 授权许可】

Unknown