Phalloidin depletes the mitochondrial Ca2+ compartment of hepatocytes

FEBS Letters

Faulstich, Heinz¹  Münter, Klaus¹  Mayer, D.² 

[1] Department of Physiology, Max-Planck-Institute for Medical Research, Jahnstr. 29, 6900 Heidelberg FRG;Division of Cytopathology, Institute of Experimental Pathology, German Cancer Research Center, Im Neuenheimer Feld 280, 6900 Heidelberg, FRG

关键词: Phalloidin;    Mushroom toxin;    Hepatocyte;    Ca2+ pool;    Mitochondria;    FCS;    fetal calf serum;    PBS;    phosphate-buffered saline;    DMPHN;    demethylphalloin;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;   

DOI  :  10.1016/0014-5793(84)80134-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In 3 h of incubation, primary cultures of rat hepatocytes attach to the substratum and exchange about 2.2 nmol ⁴⁵Ca²⁺ per mg protein. In the presence of 1 μM phalloidin, the exchanged amount of ⁴⁵Ca²⁺ was found to be decreased by about 30%. Using the uncoupling agent FCCP and the ionophore A23187 for further characterisation we determined that the ⁴⁵Ca²⁺ deficit caused by phalloidin occurs in the FCCP-sensitive compartment, i.e., the mitochondria.

【 授权许可】

Unknown