期刊论文详细信息
FEBS Letters | |
Phalloidin depletes the mitochondrial Ca2+ compartment of hepatocytes | |
Faulstich, Heinz1  Münter, Klaus1  Mayer, D.2  | |
[1] Department of Physiology, Max-Planck-Institute for Medical Research, Jahnstr. 29, 6900 Heidelberg FRG;Division of Cytopathology, Institute of Experimental Pathology, German Cancer Research Center, Im Neuenheimer Feld 280, 6900 Heidelberg, FRG | |
关键词: Phalloidin; Mushroom toxin; Hepatocyte; Ca2+ pool; Mitochondria; FCS; fetal calf serum; PBS; phosphate-buffered saline; DMPHN; demethylphalloin; FCCP; carbonyl cyanide p-trifluoromethoxyphenylhydrazone; | |
DOI : 10.1016/0014-5793(84)80134-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In 3 h of incubation, primary cultures of rat hepatocytes attach to the substratum and exchange about 2.2 nmol 45Ca2+ per mg protein. In the presence of 1 μM phalloidin, the exchanged amount of 45Ca2+ was found to be decreased by about 30%. Using the uncoupling agent FCCP and the ionophore A23187 for further characterisation we determined that the 45Ca2+ deficit caused by phalloidin occurs in the FCCP-sensitive compartment, i.e., the mitochondria.
【 授权许可】
Unknown
【 预 览 】
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