Conversion of 1‐aminocyclopropane‐1‐carboxylic acid to ethylene in submitochondrial particles isolated from plants

FEBS Letters

Apelbaum, Akiva¹  Vinkler, Chana¹ 

[1] Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Center, PO Box 6, Bet Dagan 50250, Israel

关键词: Ethylene;    1-Aminocyclopropane-1-carboxylic acid;    Submitochondrial particle;    Inhibitor;    Substrate analog;    SMP;    submitochondrial particles;    ACC;    1-aminocyclopropane-1-carboxylic acid;    2;    4 DNP;    2;    4-dinitrophenol;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;    KMB;    α-keto-γ-methylthiobutyric acid;    Hepes;    4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid;    Pipes;    1;    4-piperazinediethanesulfonic acid;    CAPS;    3-(cyclohexylamino)propanesulfonic acid;   

DOI  :  10.1016/0014-5793(84)80833-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene was studied in submitochondrial particles (SMP) which were prepared by sonication of the mitochondrial fraction isolated from 7-day-old etiolated pea (Pisum sativum var. Kelvedon Wonder) seedlings. The reaction was enhanced by the addition of Mn²⁺ and had a maximal rate at pH 8.0. Conversion of ACC to ethylene was inhibited under anaerobic conditions and by the addition of KCN, EDTA, NaN₃, n-propyl gallate and CoCl₂. Addition of the uncouplers 2,4-DNP, CCCP and FCCP, however, did not inhibit the reaction. Structural analogs of ACC inhibited ACC conversion to enthylene by SMP. The structural analog of methionine, α-keto-γ-methylthiobutyric acid (KMB), was converted to ethylene by SMP at a rate which was only about 2% that of ACC conversion to ethylene.

【 授权许可】

Unknown