On a non‐pyridine nucleotide‐dependent 2‐oxo‐acid reductase of broad substrate specificity from two Proteus species

FEBS Letters

Simon, Helmut¹  Neumann, Stefan¹ 

[1] Lehrstuhl für Organische Chemie und Biochemie, TU München, Lichtenbergstr. 4, 8046 Garching, FRG

关键词: Proteus mirabilis;    Proteus vulgaris;    2-Oxo-carboxylic acid reductase;    (2R)-Hydroxy-acid preparation;    Viologen;    methylviologen or MV2+;    1;    1′-dimethyl-4;    4′-dipyridinium dication;    MV+·;    reduced methylviologen;    BV2+;    benzylviologen;    BV+·;    reduced benzylviologen;   

DOI  :  10.1016/0014-5793(84)80826-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteus mirabilis and Proteus vulgaris contain in the crude extract a non-pyridine nucleotide dependent reductase for 2-oxo-acids as well as 2-oxo-dicarboxylic acids of specific activities of 1–10 units/mg protein. The enzyme catalyzes the reduction of very different 2-oxo-acids to the corresponding (2R)-hydroxy-acid. Reduced methyl- or benzylviologen act as artifical electron donors. The membrane-bound enzyme has been enriched 167-fold to a purity 90% as judged by electrophoresis. It seems that the enzyme does not contain flavin.

【 授权许可】

Unknown