Effects of hypothyroidism on myosin isozyme transitions in developing rat muscle

FEBS Letters

Herlicoviez, Danielle¹  Butler-Browne, Gillian S.²  Whalen, Robert G.² 

[1] Laboratoire de Neuropathologie, CHU Côte de Nacre, 14040 Caen, France;Département de Biologie Moléculaire, Institut Pasteur, 25, Rue du Dr. Roux, 75724 Paris, France

关键词: Immunocytochemistry;    Native myosin isozyme;    Methylthiouracil;    Soleus;    Gastrocnemius;   

DOI  :  10.1016/0014-5793(84)80047-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hypothyroidism was induced in young rats by methylthiouracil treatment of pregnant mothers from 18 days of gestation to 4 weeks after birth. Electrophoretic analysis of native myosin isozymes revealed a persistence of neonatal and embryonic myosin in developing fast and slow muscles up to at least 28 days after birth. The appearance of adult fast myosin was inhibited in 28-day old animals, however adult slow myosin was found in the soleus muscle. Immunocytochemical results on the soleus demonstrate a cellular heterogeneity in the response to hypothyroidism. About half fibers have a normal complement of slow myosin and do not contain neonatal myosin. Only the remaining fibers contain the large amounts of neonatal myosin demonstrated by electrophoresis.

【 授权许可】

Unknown