Expression of human α1‐antitrypsin in Escherichia coli

FEBS Letters

Herzog, Albert¹  Bollen, Alex¹  Loriau, Rosette¹  Hérion, Pascal¹ 

[1]Department of Molecular Biology, University of Brussels, 67 rue des Chevaux, B 1640 Rhode-St-Genèse, Belgium

关键词: Human α1-antitrypsin;    Plasma protein;    Recombinant DNA;    Cloning of human cDNA;    Bacterial expression of human cDNA;    Immunoassay for α1-antitrypsin;   

DOI  :  10.1016/0014-5793(84)80046-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Complementary DNA coding for human α₁-antitrypsin has been placed under the control of the λP_R promotor carrier by the expression vector pCQV2 [1]. In conditions which allow transcription from this promotor (thermoinactivation of the repressor), Escherichia coli cells harbouring the recombinant plasmid pULB1114 express human α₁-antitrypsin (± 9000 molecules/cell). The product has a M _r of 44 000, corresponding to mature unglycosylated α₁-antitrypsin.

【 授权许可】

Unknown