Effects of proteolysis on the actions of monovalent cations and 1‐O‐octadecyl‐2‐O‐acetyl‐sn‐glyceryl‐3‐phosphorylcholine on platelet adenylate cyclase

FEBS Letters

Haslam, Richard J.¹  Williams, Kimberley A.¹  Vanderwel, Marianne¹ 

[1] Department of Pathology, McMaster University, Hamilton, Ontario L8N 3Z5, Canada

关键词: Adenylate cyclase;    Proteolysis;    NaCl;    1-O-octadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine;    1-octadecyl-2-acetyl-G-3-PC;    1-O-octadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine;    EGTA;    ethylene glycol bis(β-aminoethyl ether)-N;    N;    N′;    N′-tetraacetic acid;   

DOI  :  10.1016/0014-5793(84)80035-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NaCl stimulated the adenylate cyclase activities of human and rabbit platelet particulate fractions prepared in the presence of ethylene glycol bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetate, but inhibited the activities of particulate fractions proteolysed by endogenous Ca²⁺-activated protease or treatment with α-chymotrypsin. Studies with other monovalent cations showed that LiCl had weak effects similar to those of NaCl, whereas KCl inhibited the enzyme in both proteolysed and non-proteolysed preparations. The results suggest that NaCl exerts stimulatory and inhibitory effects through different sites. NaCl potentiated and proteolysis greatly reduced the inhibition of platelet adenylate cyclase by 1-O-octadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine (platelet-activating factor).

【 授权许可】

Unknown