Inhibition of glycogenolysis by 2,5‐anhydro‐D‐mannitol in isolated rat hepatocytes

FEBS Letters

Stevens, Hope C.²  Dills, William L.¹ 

[1] Section of Biochemistry, Cell and Molecular Biology, Division of Biological Sciences, Cornell University, Ithaca, NY, 14853 USA;Division of Nutritional Sciences, Cornell University, Ithaca, NY, 14853, USA

关键词: 2;    5-Anhydro-D-mannitol;    Glycogenolysis;    Glucose synthesis;    Glycogen phosphorylase;    Heriditary fructose intolerance;    Hepatocyte;    AM;    2;    5-anhydro-D-mannitol;    AM-1-P;    2;    5-anhydro-D-mannitol-1-phosphate;    AMBP;    2;    5-anhydro-D-mannitol-1;    6-biphosphate;   

DOI  :  10.1016/0014-5793(84)80178-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

2,5-Anhydro-D-mannitol, an analog of D-fructofuranose, inhibited basal and glucagon-stimulated glycogenolysis and glucose production in hepatocytes isolated from fed rats. Glucose formation from galactose was unaffected by the inhibitor. 2,5-Anhydro-D-mannitol-1-phosphate inhibits phosphorylase a with a K _i value of 2.4mM. This same phosphorylated metabolite accumulates to the extent of 9.2 μmol/g wet wt in treated hepatocytes suggesting that phosphorolysis is the locus of the inhibition of glucose production from glycogen. Our results suggest that 2,5-anhydro-D-mannitol can be used to produce a model of hereditary fructose intolerance and that it merits further study as a hypoglycemic agent.

【 授权许可】

Unknown