Raman spectroscopic analysis of the secondary structure in Panulirus interruptus hemocyanin

FEBS Letters

Williams, Robert W.²  Hendrickson, Wayne A.¹ 

[1] Laboratory for the Structure of Matter, code 6030, Naval Research Laboratory, Washington, DC 20375, USA;Optical Probes Branch, code 6510, Naval Research Laboratory, Washington, DC 20375, USA

关键词: Secondary structure;    Raman spectroscopy;    Hemocyanin;   

DOI  :  10.1016/0014-5793(84)80173-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The secondary structure content of Panulirus interruptus hemocyanin crystals is estimated to be 29±5% helix and 42±4% ß-strand. These estimates are obtained from an analysis of the Raman amide I spectrum. This report constitutes a test of this method of analyzing Raman spectra since the X-ray structure of P. interruptus, which is unknown to us, is under intensive study. Also, the amide I spectrum of P. interruptus in solution, at a somewhat higher pH, is significantly different from the spectrum of crystals which indicates that the secondary structure content may be different in the solution sample.

【 授权许可】

Unknown