FEBS Letters | |
The complete amino acid sequence of hirudin, a thrombin specific inhibitor | |
Chang, Jui-Yoa3  Dodt, Johannes2  Seemüller, Ursula2  Müller, Hans-Peter1  | |
[1] Plantorgan Werk KG, Bad Zwischenahn, FRG;Department of Clinical Chemistry and Clinical Biochemistry, University of Munich, Munich, Switzerland;Pharmaceuticals Research Laboratories, CIBA-GEIGY Ltd, Basel, Switzerland | |
关键词: Hirudin; Tyrosine sulfate; Color carboxymethylation; DABIA; 4-dimethylaminobenzene 4-iodoacetamide; DABITC; dimethylaminoazobenzene isothiocyanate; DABS-Cl; dimethylaminoazobenzene sulfonyl chloride; HPLC; high-performance liquid chromatography; PTH; phenylthiohydantoin; | |
DOI : 10.1016/0014-5793(84)80165-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Color carboxymethylation of cysteine residues with a new chromophoric reagent dimethylaminoazobenzene iodoacetamide, was applied to the micro-sequence analysis of hirudin, a thrombin specific inhibitor. Six cysteine residues of the reduced hirudin were detected as colored phenylthiohydantoin derivative and 3 tryptic peptides of hirudin (all containing cysteines) were isolated as colored peptide. The complete hirudin sequence, including 6 uncertain positions left in the previous report [Petersen T.E. (1976) in: Protides of the Biological Fluids; 23rd Colloquium, pp. 145, Pergamon Press, London] was established.
【 授权许可】
Unknown
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