The complete amino acid sequence of hirudin, a thrombin specific inhibitor

FEBS Letters

Chang, Jui-Yoa³  Dodt, Johannes²  Seemüller, Ursula²  Müller, Hans-Peter¹ 

[1] Plantorgan Werk KG, Bad Zwischenahn, FRG;Department of Clinical Chemistry and Clinical Biochemistry, University of Munich, Munich, Switzerland;Pharmaceuticals Research Laboratories, CIBA-GEIGY Ltd, Basel, Switzerland

关键词: Hirudin;    Tyrosine sulfate;    Color carboxymethylation;    DABIA;    4-dimethylaminobenzene 4-iodoacetamide;    DABITC;    dimethylaminoazobenzene isothiocyanate;    DABS-Cl;    dimethylaminoazobenzene sulfonyl chloride;    HPLC;    high-performance liquid chromatography;    PTH;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(84)80165-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Color carboxymethylation of cysteine residues with a new chromophoric reagent dimethylaminoazobenzene iodoacetamide, was applied to the micro-sequence analysis of hirudin, a thrombin specific inhibitor. Six cysteine residues of the reduced hirudin were detected as colored phenylthiohydantoin derivative and 3 tryptic peptides of hirudin (all containing cysteines) were isolated as colored peptide. The complete hirudin sequence, including 6 uncertain positions left in the previous report [Petersen T.E. (1976) in: Protides of the Biological Fluids; 23rd Colloquium, pp. 145, Pergamon Press, London] was established.

【 授权许可】

Unknown