Immunocytochemical localization of the elongation factor Tu in E. coli cells

FEBS Letters

Bosch, Leendert²  Cremers, Alfons F.M.²  Posthuma, George¹  van der Meide, Peter H.²  Schilstra, Maria J.²  Slot, Jan W.¹ 

[1] State University of Utrecht, Faculty of Medicine, Centre for Electron Miscrocopy, 22 Nic. Beetsstraat, 3511HG Utrecht, The Netherlands;State University of Leiden, Department of Biochemistry, 64 Wassenaarseweg, 2333 AL Leiden, The Netherlands

关键词: Elongation factor Tu;    E. coli;    Immunoelectron microscopy;    Ultracryotomy;    Colloidal gold labelling;    EF-Tu;    elongation factor Tu;   

DOI  :  10.1016/0014-5793(84)80164-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The localization of the elongation factor Tu (EF-Tu) in ultrathin cryosections of E. coli cells was determined with the electron microscope using a highly specific immunological labellin technique. EF-Tu is distributed almost homogeneously throughout the cytoplasm. Although it has often been suggested that EF-Tu could be part of a putative prokaryotic cytoskeleton, we did not find any evidence for supramolecular assemblies, such as fibres or filaments, containing a large amount of EF-Tu. EF-Tu was not observed in association with the outer cell membrane and periplasmic space. A topological relationship with the inner membrane is not apparent in our micrographs. In cells in which the EF-Tu level is raised significantly, the protein piles up in discrete cell regions.

【 授权许可】

Unknown