Reconstitution of light‐harvesting chlorophyll‐protein complexes with Photosystem II complexes in soybean phosphatidylcholine liposomes

FEBS Letters

Woodrow, Ian E.²  Crowther, David¹  Murphy, Denis J.¹ 

[1] ARC Research Group on Photosynthesis, Department of Botany, The University, Sheffield S10 2TN, England;Department of Environmental Biology, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia

关键词: Reconstitution;    Photosystem II complex;    Light-harvesting chlorophyll-protein complex;    Proteoliposome;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    DCPIP;    2;    6-dichlorophenolindophenol;    DPC;    diphenylcarbazide;    LHCP;    light-harvesting chlorophyll-protein;    PS;    photosystem;    Chlorophyll;    Chl;   

DOI  :  10.1016/0014-5793(84)80160-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isolated Photosystem II (PS II) complexes and light-harvesting chlorophyll-protein complexes have been reconstituted, both separately and together, into soybean phosphatidylcholine liposomes. PS II activity, as measured by dichlorophenolindophenol reduction, increased by 70% following reconstitution of PS II complexes. Proteoliposomes containing both PS II and light-harvesting chlorophyll-protein complexes also exhibited enhanced quantum efficiencies at sub-saturating light intensities. This indicates that the light-harvesting complexes are interacting with the PS II complexes in proteoliposomes, to increase effectively their antenna size.

【 授权许可】

Unknown