Study of the binding of jatrophone to Escherichia coli s‐ribonucleic acid

FEBS Letters

D'Alagni, Maria¹  Temussi, Piero Andrea²  De Petris, Marco¹  Battista Marini-Bettolo, Giovanni¹ 

[1] Centro di Studio per la Chimica dei Recettori e delle Molecole Biologicamente Attive del C.N.R., c/o Istituto de Chimica, Università Cattolica, Via della Pineta Sacchetti 644, 00168 Roma, Italy;Dipartimento di Chimica, Università di Napoli, Via Mezzocannone 4, 80134 Napoli, Italy

关键词: Jatrophone;    sRNA binding;    Aqueous;    Solution;    UV;    CD;    1H NMR;   

DOI  :  10.1016/0014-5793(83)80017-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of jatrophone with sRNA with sRNA from Escherichia coli has been investigated through UV, CD, and ¹H NMR measurements. The results obtained show that the interaction with jatrophone increases the stability of the polynucleotide. It appears that the optical properties of jatrophone depend upon the jatrophone/nucleotide ratio. The observed behaviour can only be explained by the existence of different types of interaction between jatrophone and sRNA. Even for a jatrophone/nucleotide ratio as low as 0.17 the ¹H NMR spectra show a multiplicity of resonances that can only be explained by the simultaneous existence of two different binding modes involving the jatrophone molecules.

【 授权许可】

Unknown