Diazepam inhibits cell respiration and induces fragmentation of mitochondrial reticulum

FEBS Letters

Zorov, Dmitry B.¹  Vorobjev, Ivan A.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, 117234 Moscow, USSR

关键词: Diazepam;    Mitochondrial reticulum;    Cell culture;    Respiration;    Membrane potential;    Fluorescent probe;   

DOI  :  10.1016/0014-5793(83)80842-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Diazepam (70–150 μg/ml) significantly inhibits oxygen consumption by pig kidney embryo cells and causes the cellular ATP level to fall. The maximum inhibitory effect develops after 1.5–2.5 h of diazepam treatment. In isolated mitochondria diazepam inhibits respiration in state 2 and 3_u with glutamate and in state 3_u with succinate. Ethylrhodamine staining and electron microscopic study reveal fragmentation of mitochondria in living cells.

【 授权许可】

Unknown