Acetoacetate: a major substrate for the synthesis of cholesterol and fatty acids by isolated rat hepatocytes

FEBS Letters

Lopes-Cardozo, M.²  Edmond, J.¹  Geelen, M.J.H.² 

[1] Department of Biological Chemistry, School of Medicine, University of California, Los Angeles, CA 90024, USA;Laboratory of Veterinary Biochemistry, State University of Utrecht, Utrecht, The Netherlands

关键词: Isolated hepatocyte;    Acetoacetate utilization;    Lipogenesis;    Cholesterogenesis;    Fatty acid synthesis;    Ketone bodies;   

DOI  :  10.1016/0014-5793(83)80833-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Evidence is presented that isolated, intact rat hepatocytes can synthesize fatty acids and cholesterol from acetoacetate. The quantitative importance of these processes is evaluated by measuring total rates of fatty acid and cholesterol synthesis by incorporation of ³H from ³H₂O. The contribution of acetoacetate varies from 14–54% and from 21–75% for de novo synthesized fatty acids and cholesterol, respectively, depending on the physiological condition of the donor rat. The relative contribution of acetoacetate to cholesterol synthesis is 1.4–2.3-times greater than to fatty acid synthesis.

【 授权许可】

Unknown