Classification of tropomyosin components into an α‐like or a β‐like family by partial peptide mapping

FEBS Letters

Fiszman, Marc-Yves¹  Kardami, Elissavet¹ 

[1] Institut Pasteur, Biologie Moléculaire, 28 Rue du Dr Roux, 75724 Paris Cedex 15, France

关键词: Tropomyosin classification;    Partial peptide mapping;    β-Tropomyosin ‘difference’ peptide;    SDS;    sodium dodecyl sulphate;    PAGE;    polyacrylamide gel electrophoresis;    2-D;    two-dimensional;    ALD;    anterior latissimus dorsi;    PLD;    posterior latissimus dorsi;    NP-40;    Nonidet-P40 (BDH);   

DOI  :  10.1016/0014-5793(83)80829-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tropomyosins can be classified as belonging to an α-like or a β-like family depending on the absence or presence, respectively, in their protease-V8 digestion pattern of two peptides with an apparent molecular mass of 21 kDa. Chicken cardiac tropomyosin and the 43 kDa component from gizzard tropomyosin are accordingly classified as α-like tropomyosins, while the 33 kDa gizzard tropomyosin component is a β-like tropomyosin. The 21 kDa peptides have an overall charge which is more positive than that of the intact tropomyosin or any other tropomyosin peptide and probably contain the -NH₂ half of the molecule.

【 授权许可】

Unknown