Regulation of yeast phosphorylase by phosphorylase kinase and cAMP‐dependent protein kinase

FEBS Letters

Becker, Jörn Ullrich¹  Wingender-Drissen, Ruth² 

[1] Kettelhack Riker Pharma GMBH, 4820 Borken, FRG;Botanisches Institut der Universität Bonn, Kirschallee 1, 5300 Bonn 1, FRG

关键词: Yeast;    Phosphorylase;    Phosphorylase kinase;    Cyclic AMP-dependent protein kinase;   

DOI  :  10.1016/0014-5793(83)81156-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Yeast phosphorylase is phosphorylated and activated by a cyclic AMP-independent protein kinase (called phosphorylase kinase) and a cyclic AMP-dependent protein kinase. Only in the presence of both kinases is phosphorylase fully activated and phosphorylated. No evidence was found for the presence of two phosphorylation sites as an identical phosphopeptide pattern of phosphorylase is obtained after phosphorylation by either one or both kinases. The kinases probably phosphorylate identical sites but recognize different subunits of phosphorylase. Phosphorylase kinase phosphorylates the high-M _r subunit while cAMP-dependent protein kinase phosphorylates the low-M _r subunit.

【 授权许可】

Unknown